MLPA

LeanGene partners with MRC Holland to deliver MLPA products; the gold standard in copy number variation detection.

MLPA® (Multiplex Ligation-dependent Probe Amplification) is the go-to technique for studying gene copy number variations (CNVs) associated with disease. Laboratories worldwide rely on MLPA for the diagnosis and research of genetic disorders and tumours. The power of the technique lies in its versatility: MLPA can be used to detect copy number changes in anything from complete chromosomes to single exons. MLPA is also used to detect DNA methylation changes and is sensitive enough to discriminate aberrations in disease-causing genes from highly similar pseudogenes. MLPA has become the gold standard in copy number variation detection, with over 350 probemixes on the market.

Why MLPA?

CNVs play a role in hundreds of genetic disorders, and MLPA assays exist for even the rarest of them. Laboratories around the world use MLPA to ensure that deletions and duplications are adequately recognised. For disorders where CNVs make up the majority of mutations, MLPA is used as a first-line test. The same applies to disorders in which genetic analysis is complicated by pseudogenes: here, MLPA is used for its exceptional sensitivity to discriminate closely related sequences. In oncogenomics, MLPA is used to study somatic CNVs to optimise tumour profiling.

 

comparison

The importance of copy numbers in genetic diagnostics and research

Copy number variants play a role in many genetic disorders. The fast, accurate and affordable detection of CNVs is essential for clinical genetic testing and research. MRC Holland develops tools for the detection of DNA copy numbers and methylation patterns associated with disease. MRC-Holland's MLPA technique is the gold standard in this field, and is used around the world to study hereditary disorders and tumours.

principle
How Does MLPA Work?

MLPA is a simple, multiplex PCR technique that uses a single primer pair to amplify up to 60 probes, each with a unique genomic target and length. PCR amplicons are fluorescently labelled and separated and quantified by capillary electrophoresis. By comparing the resulting peak pattern of a sample to those of a set of reference samples, the number of genomic targets present in the sample of interest can be determined.

LeanGene partners with  MRC-Holland to deliver MLPA products; the gold standard in copy number variation detection.

 

MLPA┬« (Multiplex Ligation-dependent Probe Amplification) is the go-to technique for studying gene copy number variations (CNVs) associated with disease. Laboratories worldwide rely on MLPA for the diagnosis and research of genetic disorders and tumours. The power of the technique lies in its versatility: MLPA can be used to detect copy number changes in anything from complete chromosomes to single exons. MLPA is also used to detect DNA methylation changes and is sensitive enough to discriminate aberrations in disease-causing genes from highly similar pseudogenes. MLPA has become the gold standard in copy number variation detection, with over 350 probemixes on the market. MRC-Holland's MLPA technique is the gold standard in this field, and is used around the world to study hereditary disorders and tumours.

Why MLPA?

CNVs play a role in hundreds of genetic disorders, and MLPA assays exist for even the rarest of them. Laboratories around the world use MLPA to ensure that deletions and duplications are adequately recognised. For disorders where CNVs make up the majority of mutations, MLPA is used as a first-line test. The same applies to disorders in which genetic analysis is complicated by pseudogenes: here, MLPA is used for its exceptional sensitivity to discriminate closely related sequences. In oncogenomics, MLPA is used to study somatic CNVs to optimise tumour profiling.

The importance of copy numbers in genetic diagnostics and research

Copy number variants play a role in many genetic disorders. The fast, accurate and affordable detection of CNVs is essential for clinical genetic testing and research. MRC Holland develops tools for the detection of DNA copy numbers and methylation patterns associated with disease. 

MLPA Applications

Developmental & Pediatric Disorders: 

Intillictual Disabilities 

SALSA MLPA

mlpa

SALSA MLPA is the main product line for MRC-Holland with up to 60 different genomic DNA sequences can be quantified simultaneously.

MLPA products are used worldwide to study hereditary disorders, tumour profiling and methylation status in diagnostic and research. With over 350 targeted assays on the market, there is always one that meets your needs.

Data interpretation can be done using Coffalyser.Net software.

SALSA digitalMLPA

digitalmlpa

The next step in MLPA technology, where an Illumina NGS platform is used for probe sequencing and subsequent count-based probe amplicon quantification.

With digitalMLPA, up to 1000 different genomic DNA sequences can be quantified simultaneously. This enables broad, unparalleled copy number certainty in one simple reaction.

Data interpretation can be done using Coffalyser digitalMLPA software.

SALSA Melt Assays

melt assay

SALSA Melt Assays are based on high-resolution melt curve analysis, and are a fast and affordable way to identify specific allelic variants that may be associated with disease.

At present only one Melt Assay is available: SALSA MC002 SMA Newborn Screen. This assay can reliably identify 95-98% of SMA patients.

Data interpretation can be done using the melt curve analysis tools of the Q-PCR platform in use.

coffalyser net
Coffalyser.Net: MLPA analysis software

Coffalyser.Net is free MLPA analysis software made and supported by MRC Holland. Coffalyser.Net can directly import raw data files and performs advanced quality checks to increase the robustness of your MLPA results. Analysis sheets for each product can be retrieved directly fromMRC Holland's servers, ensuring you always have access to the latest versions.

coffalyser digitalmlpa
Coffalyser digitalMLPA: digitalMLPA analysis software

Coffalyser digitalMLPA is free software developed by MRC Holland for the analysis of digitalMLPA data. Coffalyser digitalMLPA automatically recognises digitalMLPA sequence reads from FASTQ files and directly uses them for analysis. Coffalyser digitalMLPA then outputs two clear reports for every sample of your experiment indicating both the quality of the run and the aberrations found.

 

 

Request Your MLPA Assay Today!

 

 

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