Cell Isolation Directly from Whole Blood!
RosetteSep™ is a fast and easy immunodensity procedure for the isolation of untouched cells directly from whole blood. By crosslinking unwanted cells to red blood cells (RBCs) present in the sample, target cells are purified during standard density gradient centrifugation.
This approach significantly reduces sample handling time and maximizes convenience by incorporating cell subset isolation into the centrifugation process.
RosetteSep™ is easily combined with SepMate™ to increase sample throughput and eliminate user errors associated with improper sample layering or PBMC harvesting. This allows users with minimal training to consistently perform cell isolation by density gradient centrifugation and is an ideal fit for a busy laboratory environment.
SepMate™ is an innovative new tube that enables consistent and hassle-free PBMC isolation in just 15 minutes. The SepMate™ insert allows users to quickly layer blood over the density gradient medium (e.g. Ficoll- Paque®, Lymphoprep™, etc.), and prevents the layers from mixing. Centrifugation is performed with the brake on, after which isolated PBMCs are simply poured off into a fresh tube. SepMate™ comes as 15ml and 50ml tubes.
There are wide range of RosetteSep™ enrichment cocktails for the isolation of highly purified cells to be used in various immunogenetics applications such as Chimerism Analysis, HLA Serological Typing, CDC Crossmatch & Flow Cytometry Crossmatch (FCXM). You have the choice of enriching Total Lymphocytes, T Cell, B Cells, Myeloid Cells, Lymphoid Cells, and many others.
