Immunomagnatic Cell Isolation

Fast, Easy and Column-Free Cell Isolation

EasySep™ is a powerful immunomagnetic cell selection procedure that combines the specifcity of monoclonal antibodies with the simplicity of a column-free magnetic system for the effortless isolation of highly purified cells. Cells are targeted for either depletion (negative selection) or selection (positive selection) using antibody complexes directed to specific cell surface antigens. The antibody complexes link targeted cells to magnetic particles. Labeled cells are pulled to the sides of the tube when the sample is placed in an EasySep™ magnet.
EasySep™ kits are available for the isolation of human T cells, B cells, granulocytes, lymphoid or myeloid cells from a variety of sample sources, including peripheral blood mononuclear cells (PBMCs), whole blood, Leuko Paks and bone marrow.

Applications like HLA Serological Typing, CDC Crossmatch Flow Cytometry Crossmatch (FCXM) & Chimerism Analysis are now easier and more accurate when combined with EasySep™ isolation kits.

Use EasySep™ to isolate cells from samples containing as few as 10 Million or as many as 4 Billion cells! 
How Does EasySep™ Work?

 

The new EasySep™ Direct kits, isolates untouched and highly purified cells directly from whole blood without density gradient centrifugation or RBC lysis. Cells are immediately ready for downstream applications including Flow Cytometry Crossmatch and Gene Expression analysis. Individual samples of 0.5 – 30 mL can be processed in as little as 20 minutes.

 
 

EasySep™ Magnets

A selection of EasySep™ Magnets is available to choose from depending on your downstream application.

 

EasySep™ Magnet

 

EasySep Magnet

Designed to hold a standard 12 x 75 mm
(5 mL) polystyrene round-bottom tube
Start Sample Cell Number Range:
0.1 - 2.5 x 108 cells
Working Volume:
(at 0.5 to 1.0 x 108 cells/mL) 0.1 - 2.5 mL


 

The BigEasy

EasySep™ Magnet

BigEasy

Designed to hold a standard 17 x 100 mm
(14 mL) polystyrene round-bottom tube
Start Sample Cell Number Range:
0.2 - 1 x 109 cells
Working Volume:
(at 0.5 to 1.0 x 108 cells/mL) 0.2 - 10 mL

 

 

The Easy 50

EasySep™ Magnet

Easy 50

Designed to hold a standard 30 x 115 mm
(50 mL) cunical tube
Start Sample Cell Number Range:
0.5 - 4 x 109 cells
Working Volume:
(at 0.5 to 1.0 x 108 cells/mL) 0.5 - 40 mL

 

The EasyEights™

EasySep™ Magnet

EasyEight

For Simultaneously Processing Multiple Samples
Designed to hold up to eight standard 5 mL round bottom tubes
(12 x 75 mm) on one side, and eight standard 14 mL round bottom
(17 x 100 mm) or 15 mL conical tubes on the other side.
Start Sample Cell Number Range:
0.125 - 2.0 x 108 cells per 5 mL tube
0.25 - 8.0 x 108 cells per 14 mL tube
Working Volume: at 0.5 to 1.0 x 108 cells/mL
0.25 - 2.0 mL per 5 mL tube
0.5 - 8.0 mL per 14 mL tube

The EasyPlate™

EasySep™ Magnet

EasyPlate

Enables Simultaneous 96-Small Volume Cell Isolations
Designed to hold a round bottom non-tissue culture treated 96-well plate
Start Sample Cell Number Range:
0.025 - 0.2 x 108 cells
Working Volume: at 0.5 to 1.0 x 108 cells/mL
0.05 - 0.2 mL

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