DSA makes it possible to screen for the presence of antibodies directed against the HLA antigens of the donor. It is ideal for pre- and post-transplant testing.
LIFECODES Donor Specific Antibody (DSA) is a bead-based immunoassay using the Luminex® for the qualitative detection of IgG antibodies to donor-specific Class I and Class II Human Leukocyte Antigens.
Human leukocyte antigens (HLA) are a system of glycoproteins that have a functional role in the presentation of peptides to the immune system. However, as a highly polymorphic system, HLA molecules can become the targets of antibody responses in people during pregnancy, transfusion of blood products, or organ transplant rejection. Generally, alloimmunization leads to the production of HLA antibodies in approximately 33% of exposed individuals. The presence or absence of these HLA-specific antibodies has a role in determining the survival of transplant allografts.
Donor Specific Antibody (DSA) provides the materials and methods required to test a serum for IgG antibody against the Class I or Class II HLA of a specific donor.
Donor lymphocytes are first isolated and lysed. The lysate is then incubated with Class I specific and Class II specific beads. The HLA antigens of the donor are bound to the specific beads via capture monoclonals. The beads are then incubated with the serum and any HLA antibodies against the donor are detected using the Luminex analyzer after addition of anti-human IgG conjugated with PE.
Features of LIFECODES DSA kit;
- Detects donor specific HLA IgG antibodies against Class I and/or Class II antigens in a single well
- Freeze donor lysate for future testing
- No cell viability issues
- Values mirror Flow Crossmatch Channel Shifts
- Run from 1 to 91 assays at a time
